Association¬ study of MCP-1 (-2518 A/G) gene polymorphisms with diabetic nephropathy in Iranian patients.


Authors:

Neda Ranjbarpour (Ms)1 ,Mahdieh Salimi (Ph.D) 2 , Hossein Mozdarani (Ph.D)3, Pegah Ghoraeian (Ph.D)1 1. Islamic Azad University Tehran Medical Branch. Tehran, Iran 2. Department of Medical Genetics, Medical Biotechnology institute, National Institute of genetic engineering and Biotechnology (NIGEB),Tehran, Iran 3. Department of Medical Genetics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

Correspondence:

salimi@nigeb.ac.ir

Aim: Monocyte chemoattractant protein-1 (MCP-1) is a chemokine that can increase adhesion molecule expression on monocytes and produce superoxide anions. Hyperglycemia induces MCP-1 production in vascular endothelial cells and retinal pigmented epithelial cells, and has been implicated as a causal factor in the facilitation of vascular complications in diabetes. Association of this polymorphisms with diabetic nephropathy has been reported in some populations in the world. In the present study, we evaluated the association of a single nucleotide polymorphism (SNP) in the MCP-1 gene with End Stage Renal Disease (ESRD) undergoing hemodialysis in Iranian patients with type 2 diabetes.

Methods: This is a case control study that was conducted in 140 unrelated patients comprising four groups: normal control, diabetic patients, ESRD cases, ESRD cases due to type two diabetes. MCP-1 genotyping of polymorphisms in the MCP-1 genes was performed using a polymerasechain reaction (PCR) followed by restriction fragment length polymorphism (RFLP)detection.

Results:Ourdata showed that there was no statistical significant association between this SNP and diabetic nephropathy but the frequency of AG genotype was more prevalent in diabetic nephropathy group compared other three groups.

Conclusion: these data showed that there was no association of mcp-1 gene polymorphism with diabetic nephropathy in Iranian patients; prospective studies with clearly-defined, homogenous cohorts are needed to confirm the effect of these genetic polymorphism of DN.

Key words: diabetic nephropathy, genetic polymorphism