Effect of endothelin antagonist on kidney ischemia –reperfusion injury in male and female rats


Nazgol Esmaelian Afyoni2 , Hanie Halili2 , Mehdi Nematbakhsh 1,2 , Fatemeh Moslemi2, Ardeshir Talebi 3 1-Department of Physiology , Faculty of Medicine , Isfahan University of Medical Sciences , Isfahan ,Iran 2-Water & Electrolytes Research Center, Isfahan University of Medical Sciences , Isfahan , Iran 3-Department of pathology , Faculty of medicine, Isfahan University of Medical Sciences , Isfahan ,Iran




Renal ischemia/reperfusion injury is the most common cause of acute kidney injury, which is associated with increased morbidity and mortality rates in clinic. Endothelin-1 receptor blockers ( such as bosentan) are recommended for the injuries caused by ischemia/reperfusion. Gender differences can affect endothelin-1 expression and the intensity of injuries caused by ischemia/reperfusion. Therefore, we hypothesized that endothelin-1 blockers may provide a gender-related effect in ischemia/reperfusion injury model in rats.


Male and female Wistar rats were assigned as sham surgery , control ischemia/reperfusion groups treated with vehicle, and case ischemia/reperfusion groups with bosentan (50 mg/kg). vehicle and bosentan were given two hours befor bilateral kidney ischemia induced by clamping renal arteries for 45 minutes followed by 24 hours of renal reperfusion.


The ischemia/reperfusion injury significantly increased the serum levels of BUN and Cr in both genders when compared with the sham group. These values were significantly decreased by bosentan in both genders. Serum levels of MDA in case ischemia/reperfusion male group were decreased significantly comparing to control ischemia/reperfusion male group.


Bosentan can be used in clinical cases inboth genders to decrease the injury caused by ischemia/reperfusion.

Keywords: Bosentan ; Kidney ; Gender ; Ischemia-Reperfusion ; Endothelin