Effect of atorvastatin on oxidative stress and renal function in experimental model of diabetic nephropathy
Mohammad Ehsan Bayatpoor1, Nastaran Faghihi2, Mohammad Taghi Mohammadi1 1. Department of Physiology and Biophysics, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran 2. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
Aim:Based on previous findings, atorvastatin has pleiotropic actions independent of lipid lowering effects such as anti-inflammation and anti-oxidant. Therefore, this study considered to examine the effects of atorvastatin on renal function and oxidative stress of renal tissue in experimental model of diabetic nephropathy.
Methods: Male Wistar rats were randomly divided into 4 groups (N=24); Normal, Normal treated, Diabetic, and Diabetic treated. The rats were made diabetic by an intravenous injection of streptozotocin (40 mg/kg) and treated rats received atorvastatin for 8 weeks (40 mg/kg/day). At the end of experiment, blood sample were collected for measurement of blood glucose and creatinine. Finally, the concentration of malondialdehyde (MDA), activity of catalase and histopathological changes were assessed.
Results: Chronic uncontrolled hyperglycemia (blood glucose above 500mg/dl) significantly increased blood creatinine of diabetic group (1.70±0.30 mg/dl) compared with normal rats (0.40±0.07 mg/dl). Also, hyperglycemia decreased the renal activity of catalase enzyme (57%) in accompany with increment of MDA concentration (0.15±0.02 nmol/mg protein) compared with normal group (0.04±0.02 nmol/mg protein). Atorvastatin significantly decreased blood creatinine of diabetic rats (0.70±0.20 mg/dl) in accompany with histopathological damages. Finally, the renal activity of catalase enzyme was increased in renal tissue (52%) concomitant reduction of MDA concentration (0.03±0.01 nmol/mg protein).
Conclusions:The findings of our study reveal that atorvastatin is able to prevent from appearance of nephropathy during chronic hyperglycemia. It is appeared that inhibition of free radical inhibition and induction of oxidative stress is a key factor that atorvastatin prevents hyperglycemia-induced nephropathy.