Association of CTLA4 polymorphisms as genetic risk factor in kidney transplant rejection


Andia Taleb 1, Mohsen Nafar2, Seyed Mohammad Hossein Ghaderian2 ,Samaneh Ghayem Amani1 , Mohammad Samzadeh 1,2, Mahdieh Imani1, Leila Ghotbi1, Mahdi Afshari3, Mandana Hasanzad1. 1. Medical Genomics Research Center, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran. 2. Urology and Nephrology Research Center (UNRC), Shahid Labbafinejad Medical Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. 3. Department of Community Medicine, Zabol University of Medical Sciences, Zabol, Iran.


Mandana Hasanzad , Email:

Background: The most effective and common treatment for end-stage renal disease is kidney transplantation. CTLA4 may be a suitable candidate gene for studying allograft rejection.

The aim of our study was to understand whether we can consider these two SNPs of CTLA4 gene as a risk factor of transplant rejection in Iranian population.

Materials and Methods: 169 kidney transplant recipients who underwent transplantation before 2010 were included in this study. The patients were classified into two groups according to Acute Rejection (AR) episodes. The -318C/T and +49A/G polymorphisms in CTLA4 gene were determined by RFLP.

Results: In +49 A/G polymorphism frequency of AG genotype was the same in both patients with and without history of rejection. None of those groups had homozygote genotype. Therefore, this polymorphism had no association with allograft rejection.

In -318C/T both CT and TT frequencies among patients whose transplantations had been rejected were lower than patients with normal outcome. Although that was not statistically significant. Compared to CC genotype as reference, TT genotype, caused 29% increase in the odds of rejection. However, this association was not significant (OR=1.29, p=1).

Conclusion: The results of our study suggest that rs231775 (+49A/G) and rs5742909 (-318C/T) CTLA4 gene polymorphisms are not linked to acute rejection in the Iranian population who underwent kidney transplantation. So these polymorphisms cannot be considered as risk factors of acute allograft rejection in Iranian renal transplantation recipients.